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Anaerobes
Fungi / Yeasts
Gram Positive
Gram Negative

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Scientific Publications - Gram Negative

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Gram negative Isolate Test Samples Gene Specificity Sensitivity Reference
AcinetobacterA. baumanniiRT-multiplex PCRblood cultureInternal transcribed spacer regions (ITS)98.80%100 CFU/ml 100% ; 30 CFU/ml 100% ; 3 CFU/ml 85%A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples, Lutz Eric Lehmann et al., Med Microbiol Immunol (2008) 197:313-324
AcinetobacterA. baumanniiDNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)No percentage given93%DNA microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
AcinetobacterA. baumanniiLightCycler® SeptiFast test MGRADEblood cultureNot Described93.50%85%Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis, Barbara Lucignano et al., J. Clin. Microbiol. doi:10.1128/JCM.02460-10 published online ahead of print on 6 April 2011
AeromonasA. hydrophilaDNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)No percentage given93%DNA Microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
BilophilaB. wadsworthiaOligonucleotide Arrayreference microorganismsBwad6-191.70%99.70%Identification of Clinically Important Anaerobic Bacteria by an Oligonucleotide Array, Yu Tzu Lin. JOURNAL OF CLINICAL MICROBIOLOGY, Apr. 2010, Vol. 48,No. 4, p. 1283-1290
BurkholderiaB. cepaciaDNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)No percentage given93%DNA Microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
CampylobacterC. jejuniDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-31
CampylobacterC. coliDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-67
CampylobacterC. coliPCRfecesinvA98%96% (cary Blair stool)Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
CampylobacterC. coliPCRfecesinvA98%87% (fresh stool)Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
CampylobacterC. jejuniPCRfecescadF98%87% (fresh stool)Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
CampylobacterC. jejuniPCRfecescadF98%96% (cary Blair stool)Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
ChlamydiaC. trachomatisLaboratory-developed quadruplex assay (LDQA)urineCryptic plasmid99.90%100%Validation of a laboratory-developed real-time PCR protocol for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine, M J Hopkins, Sex Transm Infect 2010;86:207e211. doi:10.1136/sti.2009.040634
CitrobacterC. amalonaticusDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-46
CitrobacterC. freundiiDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-47
CitrobacterC. koseriDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-48
CitrobacterC. braakiiDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-49
CitrobacterC. freundiiDNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)No percentage given93%DNA Microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
EnterobacterE. hormaecheiDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-50
EnterobacterE. sakazakiiDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-51
EnterobacterE. aerogenesRT-multiplex PCRblood cultureInternal transcribed spacer regions (ITS)98.80%100 CFU/ml 100% ; 30 CFU/ml 100% ; 3 CFU/ml 50%A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples, Lutz Eric Lehmann et al., Med Microbiol Immunol (2008) 197:313-324
EnterobacterE. cloacaeRT-multiplex PCRblood cultureInternal transcribed spacer regions (ITS)98.80%100 CFU/ml 100% ; 30 CFU/ml 100% ; 3 CFU/ml 75%A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples, Lutz Eric Lehmann et al., Med Microbiol Immunol (2008) 197:313-324
EnterobacterE. aerogenesDNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)No percentage given93%DNA Microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
EnterobacterE. cloacaeLightCycler® SeptiFast test MGRADEblood culture16S-23S and 18S-109 5.8S ITS region of rRNA genes93.50%85.00%Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis, Barbara Lucignano et al., J. Clin. Microbiol. doi:10.1128/JCM.02460-10 published online ahead of print on 6 April 2011
EnterobacterE. aerogenesLightCycler® SeptiFast test MGRADEblood culture16S-23S and 18S-109 5.8S ITS region of rRNA genes93.50%85.00%Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis, Barbara Lucignano et al., J. Clin. Microbiol. doi:10.1128/JCM.02460-10 published online ahead of print on 6 April 2011
EnterococcusE. faecalisDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-40
EnterococcusE. faeciumDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-41
EscherichiaE. coliRT-multiplex PCRblood cultureInternal transcribed spacer regions (ITS)98.80%100 CFU/ml 100% ; 30 CFU/ml 100% ; 3 CFU/ml 100%A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples, Lutz Eric Lehmann et al., Med Microbiol Immunol (2008) 197:313-324
EscherichiaE. coliPCR-ELISAreference microorganisms16S rDNANo percentage givenNo percentage givenA rapid method for the detection of representative coliforms in water samples: polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA), Jong-Tar Kuo et al., J Ind Microbiol Biotechnol (2010) 37:237-244
EscherichiaE. coliDNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)93.50%85%DNA microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
EscherichiaE. coliLightCycler® SeptiFast test MGRADEblood culture16S-23S and 18S-109 5.8S ITS region of rRNA genesNo percentage given85%Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis, Barbara Lucignano et al., J. Clin. Microbiol. doi:10.1128/JCM.02460-10 published online ahead of print on 6 April 2011
EscherichiaE. coliRT-PCRCSF16S rRNA54.0%, this specificity does not reflect the true percentage, because in many cases with a negative culture an antibiotic had been prescribed before the bacterial cultivation of the CSF.100%Rapid detection of eight causative pathogens for the diagnosis of bacterial meningitis by real-time PCR, Naoko Chiba et al., J Infect Chemother (2009) 15:92-98
EscherichiaE. coli.PCRfecesipaH98%96% (cary Blair stool)Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
EscherichiaE. coli.PCRfecesipaH98%87% (fresh stool)Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
HaemophilusH. influenzaeDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-30
HaemophilusH. aphrophilus16S rRNA gene sequencingblood culture16S rRNANo percentage givenNo percentage givenIdentification of oral bacteria in blood cultures by conventional versus molecular methods, Farah K. Bahrani-Mougeot, ORAL MEDICINE, Vol. 105No. 6 June 2008
HaemophilusH. influenzaeRT-PCRCSF16S rRNA54.0%, this specificity does not reflect the true percentage, because in many cases with a negative culture an antibiotic had been prescribed before the bacterial cultivation of the CSF.100%Rapid detection of eight causative pathogens for the diagnosis of bacterial meningitis by real-time PCR, Naoko Chiba et al., J Infect Chemother (2009) 15:92-98
KlebsiellaK. oxytocaRT-multiplex PCRblood cultureInternal transcribed spacer regions (ITS)98.80%100 CFU/ml 100% ; 30 CFU/ml 100% ; 3 CFU/ml 85%A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples, Lutz Eric Lehmann et al., Med Microbiol Immunol (2008) 197:313-324
KlebsiellaK. pneumoniaeRT-multiplex PCRblood cultureInternal transcribed spacer regions (ITS)98.80%100 CFU/ml 100% ; 30 CFU/ml 100% ; 3 CFU/ml 80%A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples, Lutz Eric Lehmann et al., Med Microbiol Immunol (2008) 197:313-324
KlebsiellaKlebsiella pneumoniaePCR-ELISAreference microorganisms16S rDNANo percentage givenNo percentage givenA rapid method for the detection of representative coliforms in water samples: polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA), Jong-Tar Kuo et al., J Ind Microbiol Biotechnol (2010) 37:237-244
KlebsiellaKlebsiella pneumoniaeDNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)No percentage given93%DNA microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
KlebsiellaKlebsiella oxytocaLightCycler® SeptiFast test MGRADEblood culture16S-23S and 18S-109 5.8S ITS region of rRNA genes93.50%85%Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis, Barbara Lucignano et al., J. Clin. Microbiol. doi:10.1128/JCM.02460-10 published online ahead of print on 6 April 2011
KlebsiellaKlebsiella pneumoniaeLightCycler® SeptiFast test MGRADEblood culture16S-23S and 18S-109 5.8S ITS region of rRNA genes93.50%85%Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis, Barbara Lucignano et al., J. Clin. Microbiol. doi:10.1128/JCM.02460-10 published online ahead of print on 6 April 2011
KluyveraK. intermediaDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-52
MoraxellaM. catarrhalisPCRreference microorganismscopBNo percentage given100%Age-related genotypic and phenotypic differences in Moraxella catarrhalis isolates from children and adults presenting with respiratory disease in 2001-2002, Suzanne J. C. Verhaegh, Microbiology (2008), 154, 1178-1184
MoraxellaM. catarrhalisPCRreference microorganismsompCDNo percentage given100%Age-related genotypic and phenotypic differences in Moraxella catarrhalis isolates from children and adults presenting with respiratory disease in 2001-2002, Suzanne J. C. Verhaegh, Microbiology (2008), 154, 1178-1184
MoraxellaM. catarrhalisPCRreference microorganismsompJNo percentage given100%Age-related genotypic and phenotypic differences in Moraxella catarrhalis isolates from children and adults presenting with respiratory disease in 2001-2002, Suzanne J. C. Verhaegh, Microbiology (2008), 154, 1178-1184
MorganellaM. morganiiDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-53
MorganellaM. morganiiDNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)No percentage given93%DNA Microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
MycoplasmaM. pneumoniaeRT-PCRCSF16S rRNA54.0%, this specificity does not reflect the true percentage, because in many cases with a negative culture an antibiotic has been prescribed before bacterial cultivation.100%Rapid detection of eight causative pathogens for the diagnosis of bacterial meningitis by real-time PCR, Naoko Chiba et al., J Infect Chemother (2009) 15:92-98
NeisseriaN. gonorrhoeamultiplex PCRreference microorganismspgi1100%100%Design of a multiplex PCR assay for the simultaneous detection and confirmation of Neisseria gonorrhoeae, Isabelle O'Callaghan, J Clin Pathol 2010;63:431e433. doi:10.1136/jcp.2009.071431
NeisseriaN. gonorrhoeamultiplex PCRreference microorganismspgm100%100%Design of a multiplex PCR assay for the simultaneous detection and confirmation of Neisseria gonorrhoeae, Isabelle O'Callaghan, J Clin Pathol 2010;63:431e433. doi:10.1136/jcp.2009.071431
NeisseriaN. gonorrhoeamultiplex PCRreference microorganismsporA100%100%Design of a multiplex PCR assay for the simultaneous detection and confirmation of Neisseria gonorrhoeae, Isabelle O'Callaghan, J Clin Pathol 2010;63:431e433. doi:10.1136/jcp.2009.071431
NeisseriaN. meningitidisRT-PCRCSF16S rRNA 54.0%, this specificty does not reflect the true percentage, because in many cases with a negative culture an antibiotic has been prescribed before bacterial cultivation.100%Rapid detection of eight causative pathogens for the diagnosis of bacterial meningitis by real-time PCR, Naoko Chiba et al., J Infect Chemother (2009) 15:92-98
NeisseriaN. gonorrhoeaLaboratory-developed quadruplex assay (LDQA)urineporA100%100%Validation of a laboratory-developed real-time PCR protocol for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine, M J Hopkins, Sex Transm Infect 2010;86:207e211. doi:10.1136/sti.2009.040634
NeisseriaN. gonorrhoeaNG-OPA/16s Duplex PCR assayurine16S rDNANo percentage given100%Validation of a laboratory-developed real-time PCR protocol for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine, M J Hopkins, Sex Transm Infect 2010;86:207e211. doi:10.1136/sti.2009.040634
NeisseriaN. gonorrhoeaNG-OPA/16s Duplex PCR assayurineOPANo percentage given100%Validation of a laboratory-developed real-time PCR protocol for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine, M J Hopkins, Sex Transm Infect 2010;86:207e211. doi:10.1136/sti.2009.040634
PantoeaP. agglomeransDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-54
PantoeaPantoea spp.DNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)No percentage given93%DNA Microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
ProteusP. mirabilisRT-multiplex PCRblood cultureInternal transcribed spacer regions (ITS)98.80%100 CFU/ml 100% ; 30 CFU/ml 100% ; 3 CFU/ml 100%A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples, Lutz Eric Lehmann et al., Med Microbiol Immunol (2008) 197:313-324
ProteusP. mirabilisLightCycler® SeptiFast test MGRADEblood culture16S-23S and 18S-109 5.8S ITS region of rRNA genes93.50%85%Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis, Barbara Lucignano et al., J. Clin. Microbiol. doi:10.1128/JCM.02460-10 published online ahead of print on 6 April 2011
ProvidenciaP. rettgeriDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-55
ProvidenciaP. stuartiiDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-56
PseudomonasP. aeruginosaRT-multiplex PCRblood cultureInternal transcribed spacer regions (ITS)98.80%100 CFU/ml 100% ; 30 CFU/ml 100% ; 3 CFU/ml 100%A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples, Lutz Eric Lehmann et al., Med Microbiol Immunol (2008) 197:313-324
PseudomonasP. aeruginosaDNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)No percentage given93%DNA microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
PseudomonasP. aeruginosaLightCycler® SeptiFast test MGRADEblood culture16S-23S and 18S-109 5.8S ITS region of rRNA genes93.50%85%Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis, Barbara Lucignano et al., J. Clin. Microbiol. doi:10.1128/JCM.02460-10 published online ahead of print on 6 April 2011
SalmonellaS. spp.RT-PCRreference microorganismsBipA100%100%A New Real-Time PCR Assay for the Specific Detection of Salmonella spp. Targeting the bipA Gene, Laia Calvó et al., Food Anal. Methods (2008) 1:236-242
SalmonellaS. spp.Multiplex PCRreference microorganismsrfbJ, wzx, fliC, fljB, wcdB sdf-I96%98%Development and evaluation of a multiplex polymerase chain reaction assay to identify Salmonella serogroups and serotypes,Nora Cardona-Castro et al., Diagnostic Microbiology and Infectious Disease 65 (2009) 327-330
SalmonellaSalmonella. spp.PNA-FISHblood cultureSal23S1098.13%94.06%Fluorescence In Situ Hybridization Method Using a Peptide Nucleic Acid Probe for Identification of Salmonella spp. in a Broad Spectrum of Samples, C. Almeida et al., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2010, p. 4476-4485
SalmonellaSalmonella. spp.PNA-FISHblood cultureSal3100%95.05%Fluorescence In Situ Hybridization Method Using a Peptide Nucleic Acid Probe for Identification of Salmonella spp. in a Broad Spectrum of Samples, C. Almeida et al., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2010, p. 4476-4485
SalmonellaSalmonella. spp.PNA-FISHblood cultureSalm6399.97%72.23%Fluorescence In Situ Hybridization Method Using a Peptide Nucleic Acid Probe for Identification of Salmonella spp. in a Broad Spectrum of Samples, C. Almeida et al., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2010, p. 4476-4485
SalmonellaSalmonella. spp.PNA-FISHblood cultureSalPNA1873100%100%Fluorescence In Situ Hybridization Method Using a Peptide Nucleic Acid Probe for Identification of Salmonella spp. in a Broad Spectrum of Samples, C. Almeida et al., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2010, p. 4476-4485
SalmonellaSalmonella spp.PCRfecesinvA8798%Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
SalmonellaSalmonella spp.PCRfecesinvA9896% (cary Blair stool)Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
SerratiaS. marcescensRT-multiplex PCRblood cultureInternal transcribed spacer regions (ITS)98.80%100 CFU/ml 100% ; 30 CFU/ml 100% ; 3 CFU/ml 100%A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples, Lutz Eric Lehmann et al., Med Microbiol Immunol (2008) 197:313-324
SerratiaS. marcescensLightCycler® SeptiFast test MGRADEblood culture16S-23S and 18S-109 5.8S ITS region of rRNA genes93.50%85%Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis, Barbara Lucignano et al., J. Clin. Microbiol. doi:10.1128/JCM.02460-10 published online ahead of print on 6 April 2011
ShigellaShigella spp.PCRfecesipaH98%96% (cary Blair stool)Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
ShigellaShigella spp.PCRfecesipaH98%87% (fresh stool)Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
SphingomonasS. paucimobilisDNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)No percentage given93%DNA Microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
StenotrophomonasStenotrophomonas maltophiRT-multiplex PCRblood cultureInternal transcribed spacer regions (ITS)98.80%100 CFU/ml 100% ; 30 CFU/ml 100% ; 3 CFU/ml 95%A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples, Lutz Eric Lehmann et al., Med Microbiol Immunol (2008) 197:313-324
StenotrophomonasS. maltophiDNA-based micro-arrayreference microorganismsbacterial 23S ribosomal DNA (rDNA) and 16S-23S intergenic spacer region (ISR)No percentage given93%DNA microarray-based identification of bacterial and fungal pathogens in bloodstream infections, Seung Min Yoo et al., Molecular and Cellular Probes 24 (2010) 44-52
StenotrophomonasS. maltophiLightCycler® SeptiFast test MGRADEblood culture16S-23S and 18S-109 5.8S ITS region of rRNA genes93.50%85%Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis, Barbara Lucignano et al., J. Clin. Microbiol. doi:10.1128/JCM.02460-10 published online ahead of print on 6 April 2011
YersiniaY. enterocoliticaDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-57
YersiniaY. pseudotuberculosisDNA-based microarray (Prove-It Sepsis Assay)blood culturegyrB, parE, mecA98.80%94.70%Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study, Päi Tissari et al., Lancet 2010; 375: 224-58
YersiniaYersinia spp.PCRfeceslysP98%96% (cary Blair stool)Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
YersiniaYersinia spp.PCRfeceslysP98%87% (fresh stool)Three-Hour Molecular Detection of Campylobacter, Salmonella, Yersinia, and Shigella Species in Feces with Accuracy as High as That of Culture, Scott A. Cunningham et al., JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2010, p. 2929-2933
EscherichiaE. coliThree color Peptide Nucleic Acid Fluorescence In Situ Hybridization AssayBlood cultureSpecies-specific ribosomal RNA (rRNA)100%98.20%Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in Blood Cultures: a Multicenter Performance Evaluation of a Three-Color Peptide Nucleic Acid Fluorescence In Situ Hybridization Assay, Della-Latta, P. et al., Journal of Clinical Microbiology 2011;49: 2259-2261
KlebsiellaK. pneumoniaeThree color Peptide Nucleic Acid Fluorescence In Situ Hybridization AssayBlood cultureSpecies-specific ribosomal RNA (rRNA)99.10%98.20%Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in Blood Cultures: a Multicenter Performance Evaluation of a Three-Color Peptide Nucleic Acid Fluorescence In Situ Hybridization Assay, Della-Latta, P. et al., Journal of Clinical Microbiology 2011;49: 2259-2261
PseudomonasP. aeruginosaThree color Peptide Nucleic Acid Fluorescence In Situ Hybridization AssayBlood cultureSpecies-specific ribosomal RNA (rRNA)95.80%98.20%Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in Blood Cultures: a Multicenter Performance Evaluation of a Three-Color Peptide Nucleic Acid Fluorescence In Situ Hybridization Assay, Della-Latta, P. et al., Journal of Clinical Microbiology 2011;49: 2259-2261
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